|
|
COSMOSIL
5PYE
Reversed-phase
HPLC columns have been widely used due to its superior resolution,
high theoretical plate numbers, ease of use and cost performance.
In reversed phase HPLC columns, hydrophobic interaction
is the dominant separation mode. For some substances similar
in hydrophobicity recognition capability may be poor, thus
resulting in insufficient separation.
The
COSMOSIL PYE reversed phase columns utilize various interactions
based on p-electrons of the stationary phase. Its stationary
phase is a planar pyrene or 2-(1-pyrenyl)ethyl) group bonded
chemically to the silica gel. Utilizing such p-p interactions
based on the pyrene ring, the PYE column separated substances
similar in structure with ease.
MATERIAL
CHARACTERISTICS |
|
COSMOSIL
5YE |
Silicagel |
totally
porous spherical silica gel |
Average
particle size |
5µm |
Average
pore size |
approx.
120Å |
Specific
surface area |
approx.
300m2/g |
Stationary
phase |
2-(1-pyrenyl)ethyl
group |
Carbon
content |
approx.
18% |
|
Hydrophobic
Interation
p-p
interaction
Charge
transfer interaction
Stereoselectivity
|
ANALYSIS
OF MONO-SUBSTITUTED BENZENE |
5PYE |
 |
Column:
4.6mm x 150mm
Mobile
phase:
5PYE
:menthanol :water: 45:55 |
5C18-MS |
 |
5C18-MS
methanol:water 50:50
Flow
rate 1.0ml/min
Detection UV 220nm, 0.6 AUFS
Sample 1. Benzene
2. Anisole
3. Nitrobenzene
|
|
 |
Column:
5PYE(4.6mmI.D.-150mm)
Mobile phase: Toluene
Flow rate: 1.0 ml/min
Detection: UV 285nm
Temperature: 30C
|
 |
|
| Perfect
separation of higher fullerenes on COSMOSIL PYE column. |
C18
columns have been commonly used for the separation of
polycyclic aromatic hydrocarbons. However, their resolution
for the separation of isomers is limited. The chromatogram
below illustrates a separation of benz[a]anthracene
and chrysene. Due to the electrostatic interaction between
the sample and the pyrene ring in the stationary phase,
the COSMOSIL PYE packed column can achieve the separation
of aromatic isomers. |
|
Column:
5PYE 4.6mmx150mm
Mobile phase: A:water, B:methanol
Gradient:B. 80%~100%, 20min linear
Flow
rate: 1.0ml/min
Detection UV 254nm,
Sample:
1. Naphthalene
2. Acenaphthylene
3. Acenapthene
4. Flourene
5. Phenanthrene
6. Anthracene
7. Fluoranthene
8. Pyrene
9. 2,3-Benzofluorene
10. Benz[a]anthracene
11. Chrysene
12. Triphenylene
13. Naphthacene
14. Benzo[a]pyrene
15. Perylene
16. Dibenz[ a,c]anthracene
17. Dibenz[ a,h]anthracen |
| Catalog
# |
Cosmosil 5PYE Column |
Price ($U.S.) |
- |
| 02851-71 |
5PYE - Semi-Microbore column, 1.0 x 150 mm |
$1,300.00 |
 |
| 093-4501 |
5PYE - Analytical Column, 2.0 x 100 mm |
$1,260.00 |
|
| 093-8042 |
5PYE - Analytical Column, 2.0 x 150 mm |
$1,200.00 |
|
| 093-4450 |
5PYE - Analytical Column, 2.0 x 250 mm |
$1,500.00 |
|
| 093-8043 |
5PYE - Analytical Column, 4.6 x 50 mm |
$900.00 |
|
| 093-7937 |
5PYE - Analytical Column, 4.6 x 150 mm |
$1,200.00 |
|
| 093-7989 |
5PYE - Analytical Column, 4.6 x 250 mm |
$1,550.00 |
|
| 093-7996 |
5PYE - Semi-preparative Column, 10.0 x 250 mm |
$5,400.00 |
|
| 093-8044 |
5PYE - Preparative Column, 20.0 x 250 mm |
$21,000.00 |
|
| 093-8101 |
5PYE - Preparative Column, 28.0 x 250 mm |
Call for Pricing |
Call |
| 093-7903 |
5PYE - Analytical Guard Column, 4.6 x 10 mm |
$400.00 |
|
| 093-7905 |
5PYE - Semi-preparative Guard Column, 10 x 20 mm |
$1,250.00 |
|
| 093-7907 |
5PYE - Preparative Guard Column, 20.0 x 20.0 mm |
$3,800.00 |
|
| 093-7906 |
5PYE - Preparative Guard Column, 20.0 x 50.0 mm |
$5,000.00 |
|
|
|
