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Cholester-D

COSMOSIL Cholester is a Reversed Phase HPLC Column with Cholestery bonded silica packing material, which provides equivalent hydrophobicity like that of traditional Alkyl group bonded silica packing materials (C18, C30). However, Cholester offers strong stereo selectivity for hydrophobic compounds to yield unique and reproducible separation patterns following the same analytical conditions used with other ODS columns.

Basic Nature
Separation of Alkylbenzene (comparison with Hydrophobicity)

Cholester provides the same hydrophobicity as Alkyl group bonded types (C18, C30). So it is not necessary to change the analytical conditions when they are replaced by Cholester.

  Cholester
Traditional C18
Traditional C30


Condition
Column size 4.6mmI.D.×150mm
Sample
1. Benzene 1.67µg
Mobile phase Methanol : Water=80 : 20 2. Toluene 1.67µg
Flow rate 1.0ml/min 3. Ethylbenzene 1.67µg
Temperature 30°C 4. Propylbenzene 1.67µg
Detection UV254nm 5. Butylbenzene 1.67µg
  6. Amylbenzene 1.67µg


Application data

The unique rigid-structure offers excellent retention and separation of difficult analyties.

Vitamin D

  Cholester Traditional C18 Traditional C30

Condition
Column size 4.6mmI.D.×150mm Sample 1. Vitamin D2 0.3µg
Mobile phase Methanol : Water=80 : 20 2. Vitamin D3 0.1µg
Flow rate 1.0ml/min  
Temperature 30°C
Detection UV265nm

Catalog # Cosmosil Cholester Column Price ($U.S.) -
5971-11 Cholester - Analytical Column, 2.0. x 150mm $464.00
5972-01 Cholester - Analytical Column, 2.0 x 250mm $505.00
5976-61 Cholester - Analytical Column, 4.6. x 150mm $464.00
5977-51 Cholester - Analytical Column, 4.6 x 250mm $505.00
5979-31 Semi-prepartive column, 10.0 x 250mm $1,262.00
5975-71 Analytical Guard Column, 4.6 x 10.0 mm $258.00
5978-41 Semi-preparative Guard Column, 10 x 20 mm $464.00
 

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