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Gel Filtration Chromatography

Gel filtration chromatography separates enzymes, proteins and other water- soluble polymers by utilizing the difference in the size of the molecules. Since mobile phases used for gel filtration chromatography are usually moderate buffer solutions or salts, this method can separate molecules while retaining their biological activity. The COSMOSIL ® Diol-II packed column is highly recommended for the separation of various water-soluble polymers.

Material characteristics
-
5DIOL-120-II
5DIOL-300-II
Silica gel
high purity spherical porous silica
Average particle size
5µm
Average pore size
approx. 120Å
approx. 300Å
Specific surface area
approx. 300m 2 /g
approx. 150m 2 /g
Stationary phase
Diol Group
Carbon content
approx.15%
approx. 9%

Selection of pore size
-
MW range
Flow rate(ml/min.)
-
protein
water soluble polymer
5DIOL-120-II
5,000-100,000
300-30,000
0.5-1.0
5DIOL-300-II
10,000-700,000
500-300,000

 

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Catalog # Cosmosil 5Diol-120 Column Price ($U.S.) -
093-8050 5Diol-120 - Packed Column, 7.5 x 300 mm $1,030.00
093-8051 5Diol-120 - Packed Column, 7.5 x 600 mm $1,545.00
093-8049 5Diol-120 - Guard Column, 7.5 x 50 mm $484.00

Catalog # Cosmosil 5Diol-300 Column Price ($U.S.) -
093-8053 5Diol-300 - Packed Column, 7.5 x 300 mm $1,030.00
093-8054 5Diol-300 - Packed Column, 7.5 x 600 mm $1,545.00
093-8052 5Diol-300 - Guard Column, 7.5 x 50 mm $484.00

 

 

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