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Gel Permeation Chromatography
Non-aqueous GPC column

High speed & high resolution
COSMOSIL ® non-aqueous GPC columns 5GPC-60, 5GPC-100 and 5GPC-300 can provide high resolution, high-speed analysis. GPC packing materials are 5µm diameter particles of totally porous spherical silica gel. Without swelling they display excellent stability against pressure.
For GPC, all samples are eluted within t0. The t0 value for a COSMOSIL ® 5GPC 4.6mmI.D.x250mm column is about three minutes at a flow rate of 1.0ml/min; meaning that the analysis is completed within about three minutes. COSMOSIL ® 5GPC column therefore require less solvent. This is important, especially when expensive solvents such as 1,1,1,3,3,3-hexafluoro-2-propanol are used. In each molecular weight range, the GPC columns provide linear correlation between log MW and the elution volume. The large pore volume results in high peak capacity. Higher efficiencies are obtained with multiple columns. Coupled columns (GPC-60 and GPC-100, or GPC-100 and GPC-300) are frequently employed to meet the wide molecular weight range of samples.
Column characteristics

Type
Particle size
Pore size
MW range
COSMOSIL ® 5GPC-60 5µm 60Å 300-15,000
COSMOSIL ® 5GPC-100 5µm 100Å 1000-50,000
COSMOSIL ® 5GPC-300 5µm 300Å 17,000-300,000

 

Recommended solvents and optimal temperature
 
- Solvents
Saturated hydrocarbons and the solvents specified below can be used with the GPC column. The use of solvents and mobile phases containing water should be avoided in order to prevent deterioration of column efficiency.
Solvents from the halogen series may corrode the stainless steel tubing and fittings of the HPLC system. After using these solvents, wash the column with solvent not containing halogen ions. Column washing and changing of mobile phase should be conducted with low flow rate. Flow rate of less than 0.5ml/min for 4.6mmI.D. columns and less than 1.5ml/min for 10mmI.D. columns are recommended.
-Temperature
Run the separation at a column temperature of 50°C lower than the boiling point of the mobile phase in order to prevent bubble formation.
Organic solvents for non-aqueous GPC columns
 

 
Physical property of the solvent
Toxicity
SOLVENT
Boiling point
°C
Viscosity
(20°C)cp.
Refractive index(20°C)
Flash Point°C
LD 50 mg/kg
L(ct) 50 ppm
1,1,1,3,3,3- Hexafluoro -2- propanol
59
-
1.2700
-
-
-
Tetrahydrofuran
66
0.51(25°C)
1.4070
14
hazardous
200
1,2,4- Trichlorobenzene
213
1.89(25°C)
1.5717
99
hazardous
75
o- Dichlorobenzene
180
1.26
1.5515
66
500
50
Toluene
110.6
0.59
1.4961
4
hazardous
6000-200
N,N- Dimethylformamide
153
0.90
1.4280
58
hazardous
100-10
Dichloromethane
40.1
0.30
1.4242
-
3000
500
1,2- Dichloroethane
84
0.84
1.4443
13
770
50
N- Methylpyrrolidone
202
1.65
1.47
95.4
hazardous
-
m- Cresol
202.8
20.8
1.544
94
hazardous
5
Benzene
80.1
0.652
1.5011
27
7000
25
Dimethyl Sulioxide
189
-
1.477
95
hazardous
-
Tetrachloroethylene
121
-
1.505
-
hazardous
100
o- Chlorophenol
175.5
4.11
1.5473
-
670
dangerous
CarbonTetrachloride
76.8
0.969
1.4607
-
hazardous
10
Trifluoroethanol
73.6
1.20
(38°C)
1.291
40.6
240
4600
Chloroform
61.7
0.58
1.446
-
hazardous
50
Acetone
56.2
0.316
1.359
-17.8
4-8
500

 

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