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COSMOSIL 5PYE

Reversed-phase HPLC columns have been widely used due to its superior resolution, high theoretical plate numbers, ease of use and cost performance. In reversed phase HPLC columns, hydrophobic interaction is the dominant separation mode. For some substances similar in hydrophobicity recognition capability may be poor, thus resulting in insufficient separation.

The COSMOSIL PYE reversed phase columns utilize various interactions based on p-electrons of the stationary phase. Its stationary phase is a planar pyrene or 2-(1-pyrenyl)ethyl) group bonded chemically to the silica gel. Utilizing such p-p interactions based on the pyrene ring, the PYE column separated substances similar in structure with ease.

MATERIAL CHARACTERISTICS
COSMOSIL 5YE
Silicagel
totally porous spherical silica gel
Average particle size
5µm
Average pore size
approx. 120Å
Specific surface area
approx. 300m2/g
Stationary phase
2-(1-pyrenyl)ethyl group
Carbon content
approx. 18%

Hydrophobic Interation
p-p interaction
Charge transfer interaction
Stereoselectivity

ANALYSIS OF MONO-SUBSTITUTED BENZENE
5PYE

Column: 4.6mm x  150mm

 

Mobile phase:

5PYE :menthanol :water: 45:55

5C18-MS

5C18-MS methanol:water 50:50

Flow rate 1.0ml/min
Detection UV 220nm, 0.6 AUFS
Sample 1. Benzene
            2. Anisole

            3. Nitrobenzene

 


Column: 5PYE(4.6mmI.D.-150mm)
Mobile phase: Toluene
Flow rate: 1.0 ml/min
Detection: UV 285nm
Temperature: 30C

 

Perfect separation of higher fullerenes on COSMOSIL PYE column.

 

SEPARATION OF PAHS
C18 columns have been commonly used for the separation of polycyclic aromatic hydrocarbons. However, their resolution for the separation of isomers is limited. The chromatogram below illustrates a separation of benz[a]anthracene and chrysene. Due to the electrostatic interaction between the sample and the pyrene ring in the stationary phase, the COSMOSIL PYE packed column can achieve the separation of aromatic isomers.

Column: 5PYE 4.6mmx150mm
Mobile phase: A:water, B:methanol

Gradient:B. 80%~100%, 20min linear

Flow rate: 1.0ml/min
Detection UV 254nm,
Sample: 
 1. Naphthalene
 2. Acenaphthylene
 3. Acenapthene
 4. Flourene
 5. Phenanthrene
 6. Anthracene
 7. Fluoranthene
 8. Pyrene
 9. 2,3-Benzofluorene
10. Benz[a]anthracene
11. Chrysene
12. Triphenylene
13. Naphthacene
14. Benzo[a]pyrene
15. Perylene
16. Dibenz[ a,c]anthracene
17. Dibenz[ a,h]anthracen

Catalog # Cosmosil 5PYE Column Price ($U.S.) -
02851-71 5PYE - Semi-Microbore column, 1.0 x 150 mm $1,421.00
093-4501 5PYE - Analytical Column, 2.0 x 100 mm $1,092.00
093-8042 5PYE - Analytical Column, 2.0 x 150 mm $1,421.00
093-4450 5PYE - Analytical Column, 2.0 x 250 mm $1,772.00
093-8043 5PYE - Analytical Column, 4.6 x 50 mm $968.00
093-7937 5PYE - Analytical Column, 4.6 x 150 mm $1,318.00
093-7989 5PYE - Analytical Column, 4.6 x 250 mm $1,926.00
093-7996 5PYE - Semi-preparative Column, 10.0 x 250 mm $6,036.00
093-8044 5PYE - Preparative Column, 20.0 x 250 mm $22,815.00
093-8101 5PYE - Preparative Column, 28.0 x 250 mm $49,718.00
093-7903 5PYE - Analytical Guard Column, 4.6 x 10 mm $443.00
093-7905 5PYE - Semi-preparative Guard Column, 10 x 20 mm $1,401.00
093-7907 5PYE - Preparative Guard Column, 20.0 x 20.0 mm $4,769.00
093-7906 5PYE - Preparative Guard Column, 20.0 x 50.0 mm $5,953.00
 

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