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Cosmosil AR-300 Series

Reversed-phase HPLC is the preferred separation method for the analysis of proteins and peptides, due to the superior resolution, high theoretical plate number, good reproducibility, ease of use and favorable cost performance.

In general, trifluoroacetic acid (TFA) is more often used as an acid mobile phase, in the analysis of proteins and peptides. However, reversed-phase HPLC using conventional silica gel shows some degradation over time in these very acidic conditions. The COSMOSIL AR-300 series packed columns have succeeded in improving acid resistance due largely to the development of a new synthesis method. It can be used for a longer time, while providing sharp peak shape even in the concentrations of 0.1% of trifluoroacetic acid.

Acid resistance

The COSMOSIL AR-300 series packed column show superior stability in the following decomposition test under the strong acidic conditions.

Capacity factor: toluene, Mobile phase :60% methanol

Types

Three alkyl and one aromatic type stationary phases are available in the COSMOSIL AR-300 series packed column. Please consult the table and applications below for selection guidance.

Alkyl type: 5C18-AR-300, 5C8-AR-300, 5C4-AR-300
Aromatic type: 5Ph-AR-300

MATERIAL CHARACTERISTICS

5C18-AR-300
5C8-AR-300
5C4-AR-300
5Ph-AR-300
Silica gel
high purity spherical porous silica
Average particle size
approx. 300Å
Specific surface area
approx. 150m²/g
Stationary
phase
octadecyl
group
octyl
group
butyl
group
phenyl
group
End capping treatment
near-perfect treatment
Carbon content
approx. 12%
approx. 7%
approx. 6%
approx. 7%

Semi-prep separation proteins

The sample loading capacity for protein or polypeptide separations is proportional to the cross section of column inside diameter. Using a short column, one can load a relatively large quantity of sample due to the absorption and concentration of sample inside the column. Below examples are shown for the separation using COSMOSIL 5C18-AR-300, with 4.6mmI.D. x 150mm column, 10mmI.D. x 150mm and 10mmI.D. x 250mm columns.
Identical separation patterns are achieved by adjusting proportionally the flow rate and the sample injection volume to the cross section of the column. There is little difference between 10mmI.D. x 150mm column and 10mmI.D. x 250mm column. Thus, semi-prep separations may be achieved using a shorter column.

Catalog # Cosmosil 5C18 -AR-300 Column Price ($U.S.) -
093-7921 5C18 -AR-300 - Analytical Column, 2.0 x 150 mm $422.00
093-7911 5C18 -AR-300 - Analytical Column, 4.6 x 50 mm $268.00
093-7912 5C18 -AR-300 - Analytical Column, 4.6 x 100 mm $453.00
093-7913 5C18 -AR-300 - Analytical Column, 4.6 x 150 mm $422.00
093-7914 5C18 -AR-300 - Analytical Column, 4.6 x 250 mm $525.00
093-7915 5C18 -AR-300 - Semi-preparative Column, 6 x 150 mm $1,071.00
093-7916 5C18 -AR-300 - Semi-preparative Column, 6x 250 mm $1,112.00
093-7917 5C18 -AR-300 - Preparative Column, 10 x 150 mm $824.00
093-7918 5C18 -AR-300 - Preparative Column, 10 x 250 mm $1,051.00
093-7919 5C18 -AR-300 - Preparative Column, 20 x 150 mm $1,648.00
093-7920 5C18 -AR-300 - Preparative Column, 20 x 250 mm $2,101.00
093-7910 5C18 -AR-300 - Analytical Guard Column, 4.6 x 10.0 mm $232.00
093-7965 5C18 -AR-300 - Semi-preparative Guard Column, 10 x 20 mm $371.00

Catalog # Cosmosil 5C8-AR-300 Column Price ($U.S.) -
093-7951 5C8-AR-300 - Analytical Column, 4.6.x 50 $330.00
093-7952 5C8-AR-300 - Analytical Column, 4.6 x 100 mm $453.00
093-7953 5C8-AR-300 - Analytical Column, 4.6 x 150 mm $422.00
093-7954 5C8-AR-300 - Analytical Column, 4.6 x 250 mm $525.00
093-7950 5C8-AR-300 - Analytical Guard Column, 4.6 x 10.0 mm $309.00

Catalog # Cosmosil 5C4-AR-300 Column Price ($U.S.) -
093-7956 5C4-AR-300 - Analytical Column, 4.6 x 50 mm $330.00
093-7957 5C4-AR-300 - Analytical Column, 4.6 x 100 mm $433.00
096-7958 5C4-AR-300 - Analytical Column, 4.6 x150 mm $494.00
093-7959 5C4-AR-300 - Analytical Column, 4.6 x 250 mm $618.00
093-8047 5C4-AR-300 - Semi-preparative Column, 10 x 250 mm $1,195.00
093-8048 5C4-AR-300 - Preparative Column, 20 x 250 mm $2,369.00
093-7955 5C4-AR-300 - Analytical Guard Column, 4.6 x 10 mm $247.00

Catalog # Cosmosil 5Ph-AR-300 Column Price ($U.S.) -
093-7961 5Ph-AR-300 - Analytical Column, 4.6.x 50 $330.00
093-7962 5Ph-AR-300 - Analytical Column, 4.6 x 100 mm $453.00
093-7963 5Ph-AR-300 - Analytical Column, 4.6 x 150 mm $453.00
093-7964 5Ph-AR-300 - Analytical Column, 4.6 x 250 mm $525.00
093-7960 5Ph-AR-300 - Analytical Guard Column, 4.6 x 10.0 mm $309.00
093-7961 5Ph-AR-300 - Analytical Column, 4.6.x 50 mm $288.00
093-7962 5Ph-AR-300 - Analytical Column, 4.6 x 100 mm $453.00
093-7963 5Ph-AR-300 - Analytical Column, 4.6 x 150 mm $422.00
093-7964 5Ph-AR-300 - Analytical Column, 4.6 x 250 mm $525.00
093-7960 5Ph-AR-300 - Analytical Guard Column, 4.6 x 10.0 mm $309.00



 

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