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Sugar-D

Conventionally Aminopropyl bounded stationary phases are used for liquid chromatographic analysis of mono- and oligosaccharides. General shortcomings of the conventional Aminopropyl bounded phases are tailing and adsorption of certain saccharides and general low durability (short active life) of these columns. These problems are addressed and solved by the novel COSMOSIL ® Sugar-D, resulting in better (sharper) separation and much improved durability.

Durability

The decrease of retention time was compared between COSMOSIL ® Sugar-D and conventional Aminopropyl bounded stationary phase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case of COSMOSIL ® Sugar-D.

Eluent condition
Eluent
Flow rate
Temperature
: water
: 1.0 ml/min
: RT

Test condition
Column size
Mobile phase
Flow rate
Temperature
Detection
Sample
: 4.6 mmI.D.×250 mm
: acetonitrile:water=70:30
: 1.0 ml/min
: 30°C
: RI
: Maltose

Absortion characteristic

Certain types of saccharides such as Arabinose or Galactose are partially or temporarily absorbed in conventional Aminopropyl stationary phases causing disappearance of sample or tailing. These problematic saccharides elute sharply and in proportion of the injection volume from the superior COSMOSIL ® Sugar-D.

Chromatogram  
Analitical curve  
Condition
Column size : 4.6 mmI.D.×250 mm
Mobile phase : acetonitrile:water=70 : 30
Flow rate : 1.0 ml/min
Temperature : 30°C
Detection : RI
Sample
1.Arabinose
2.Galactose
3.Maltitol
Comparison
The separation and adsorption performances of COSMOSIL Sugar-D and other companies columns were compared by using monosaccharides. The presence of aldehyde group in saccharides is responsible for the strong absortion causing peak tailing. Only COSMOSIL Sugar-D shows high separation performance without undesirable adsortion.

Retention Time

The conventional aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5%-10%.


Analysis
Separation of Saccharides

Analysis
Separation of highly hydrophilic compounds

Catalog # Cosmosil Sugar-D Column Price ($U.S.) -
05688-41 Sugar-D - Analytical Column, 2.0. x 150mm $464.00
05689-31 Sugar-D - Analytical Column, 2.0 x 250mm $541.00
05690-91 Sugar-D - Analytical Column, 3.0 x 150mm $525.00
05691-81 Sugar-D - Analytical Column, 3.0 x 250mm $567.00
05395-71 Sugar-D - Analytical Column, 4.6. x 150mm $464.00
05397-51 Sugar-D - Analytical Column, 4.6 x 250mm $505.00
05692-71 Sugar-D - Semi-prepartive column, 10.0 x 250mm $1,262.00
05693-61 Sugar-D - Prepartive Column, 20.0 x 250mm $2,575.00
05394-81 Sugar-D - Analytical Guard Column, 4.6 x 10.0 mm $258.00
05696-31 Sugar-D - Semi-preparative Guard Column, 10 x 20 mm $536.00
 

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