COSMOSIL Cholester is a Reversed Phase HPLC Column with Cholestery bonded silica packing material, which provides equivalent hydrophobicity like that of traditional Alkyl group bonded silica packing materials (C18, C30). However, Cholester offers strong stereo selectivity for hydrophobic compounds to yield unique and reproducible separation patterns following the same analytical conditions used with other ODS columns.
Basic Nature Separation of Alkylbenzene (comparison with Hydrophobicity)
Cholester provides the same hydrophobicity as Alkyl group bonded types (C18, C30). So it is not necessary to change the analytical conditions when they are replaced by Cholester.
Cholester
Traditional C18
Traditional C30
Condition
Column size
4.6mmI.D.×150mm
Sample
1. Benzene
1.67µg
Mobile phase
Methanol : Water=80 : 20
2. Toluene
1.67µg
Flow rate
1.0ml/min
3. Ethylbenzene
1.67µg
Temperature
30°C
4. Propylbenzene
1.67µg
Detection
UV254nm
5. Butylbenzene
1.67µg
6. Amylbenzene
1.67µg
Application data
The unique rigid-structure offers excellent retention and separation of difficult analyties.