COSMOSIL Cholester is a Reversed Phase HPLC Column with Cholestery bonded silica packing material, which provides equivalent hydrophobicity like that of traditional Alkyl group bonded silica packing materials (C18, C30). However, Cholester offers strong stereo selectivity for hydrophobic compounds to yield unique and reproducible separation patterns following the same analytical conditions used with other ODS columns.

cholestery

Basic Nature 
Separation of Alkylbenzene (comparison with Hydrophobicity)

Cholester provides the same hydrophobicity as Alkyl group bonded types (C18, C30). So it is not necessary to change the analytical conditions when they are replaced by Cholester.

  Cholester
Traditional C18
Traditional C30
basic
basic
basic
Condition
Column size4.6mmI.D.×150mm
Sample
1. Benzene1.67µgcond
Mobile phaseMethanol : Water=80 : 202. Toluene1.67µg
Flow rate1.0ml/min3. Ethylbenzene1.67µg
Temperature30°C4. Propylbenzene1.67µg
DetectionUV254nm5. Butylbenzene1.67µg
6. Amylbenzene1.67µg
 
Application data
The unique rigid-structure offers excellent retention and separation of difficult analyties.
Vitamin D
  CholesterTraditional C18Traditional C30
vitd1
vid2
vitd3
Condition
Column size4.6mmI.D.×150mmSample1. Vitamin D20.3µg
Mobile phaseMethanol : Water=80 : 202. Vitamin D30.1µg
Flow rate1.0ml/min
Temperature30°C
DetectionUV265nm
vitd2d3

 

Cosmosil Cholester Column

ItemCatalogPrice ($U.S.)Buy
Analytical Guard Column, 4.6 x 10.0 mm 5975-71 $258.00 Add to cart
Cholester – Analytical Column, 2.0 x 250mm 5972-01 $505.00 Add to cart
Cholester – Analytical Column, 2.0. x 150mm 5971-11 $464.00 Add to cart
Cholester – Analytical Column, 4.6 x 250mm 5977-51 $505.00 Add to cart
Cholester – Analytical Column, 4.6. x 150mm 5976-61 $464.00 Add to cart
Semi-preparative Guard Column, 10 x 20 mm 5978-41 $464.00 Add to cart
Semi-prepartive column, 10.0 x 250mm 5979-31 $1,262.00 Add to cart