Cholester-D

COSMOSIL Cholester is a Reversed Phase HPLC Column with Cholestery bonded silica packing material, which provides equivalent hydrophobicity like that of traditional Alkyl group bonded silica packing materials (C18, C30). However, Cholester offers strong stereo selectivity for hydrophobic compounds to yield unique and reproducible separation patterns following the same analytical conditions used with other ODS columns.

Basic Nature 
Separation of Alkylbenzene (comparison with Hydrophobicity)

Cholester provides the same hydrophobicity as Alkyl group bonded types (C18, C30). So it is not necessary to change the analytical conditions when they are replaced by Cholester.

  Cholester
Traditional C18
Traditional C30
Condition
Column size 4.6mmI.D.×150mm
Sample
1. Benzene 1.67µg
Mobile phase Methanol : Water=80 : 20 2. Toluene 1.67µg
Flow rate 1.0ml/min 3. Ethylbenzene 1.67µg
Temperature 30°C 4. Propylbenzene 1.67µg
Detection UV254nm 5. Butylbenzene 1.67µg
6. Amylbenzene 1.67µg
 
Application data
The unique rigid-structure offers excellent retention and separation of difficult analyties.
Vitamin D
  Cholester Traditional C18 Traditional C30
Condition
Column size 4.6mmI.D.×150mm Sample 1. Vitamin D2 0.3µg
Mobile phase Methanol : Water=80 : 20 2. Vitamin D3 0.1µg
Flow rate 1.0ml/min
Temperature 30°C
Detection UV265nm

 

Cosmosil Cholester Column

Item Catalog Price ($U.S.) Buy
Cholester – Analytical Column, 2.0. x 150mm 5971-11 $464.00 Add to cart
Cholester – Analytical Column, 4.6. x 150mm 5976-61 $464.00 Add to cart
Semi-preparative Guard Column, 10 x 20 mm 5978-41 $464.00 Add to cart
Semi-prepartive column, 10.0 x 250mm 5979-31 $1,262.00 Add to cart
Analytical Guard Column, 4.6 x 10.0 mm 5975-71 $258.00 Add to cart
Cholester – Analytical Column, 2.0 x 250mm 5972-01 $505.00 Add to cart
Cholester – Analytical Column, 4.6 x 250mm 5977-51 $505.00 Add to cart