COSMOSIL Cholester is a Reversed Phase HPLC Column with Cholestery bonded silica packing material, which provides equivalent hydrophobicity like that of traditional Alkyl group bonded silica packing materials (C18, C30). However, Cholester offers strong stereo selectivity for hydrophobic compounds to yield unique and reproducible separation patterns following the same analytical conditions used with other ODS columns.
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Basic Nature
Separation of Alkylbenzene (comparison with Hydrophobicity)
Cholester provides the same hydrophobicity as Alkyl group bonded types (C18, C30). So it is not necessary to change the analytical conditions when they are replaced by Cholester.
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Cholester
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Traditional C18
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Traditional C30
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Condition |
Column size |
4.6mmI.D.×150mm |
Sample
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1. Benzene |
1.67µg |
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Mobile phase |
Methanol : Water=80 : 20 |
2. Toluene |
1.67µg |
Flow rate |
1.0ml/min |
3. Ethylbenzene |
1.67µg |
Temperature |
30°C |
4. Propylbenzene |
1.67µg |
Detection |
UV254nm |
5. Butylbenzene |
1.67µg |
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6. Amylbenzene |
1.67µg |
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Application data |
The unique rigid-structure offers excellent retention and separation of difficult analyties. |
Vitamin D |
Cholester |
Traditional C18 |
Traditional C30 |
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Condition |
Column size |
4.6mmI.D.×150mm |
Sample |
1. Vitamin D2 |
0.3µg |
Mobile phase |
Methanol : Water=80 : 20 |
2. Vitamin D3 |
0.1µg |
Flow rate |
1.0ml/min |
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Temperature |
30°C |
Detection |
UV265nm |
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Cosmosil Cholester Column
Item |
Catalog |
Price ($U.S.) |
Buy |
Cholester – Analytical Column, 2.0 x 250mm
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5972-01 |
$505.00
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Add to cart |
Cholester – Analytical Column, 2.0. x 150mm
|
5971-11 |
$464.00
|
Add to cart |
Cholester – Analytical Column, 4.6. x 150mm
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5976-61 |
$464.00
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Add to cart |
Semi-preparative Guard Column, 10 x 20 mm
|
5978-41 |
$464.00
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Add to cart |
Semi-prepartive column, 10.0 x 250mm
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5979-31 |
$1,262.00
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Add to cart |
Analytical Guard Column, 4.6 x 10.0 mm
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5975-71 |
$258.00
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Add to cart |
Cholester – Analytical Column, 4.6 x 250mm
|
5977-51 |
$505.00
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Add to cart |