Reversed-phase HPLC columns have been widely used due to its superior resolution, high theoretical plate numbers, ease of use and cost performance. In reversed phase HPLC columns, hydrophobic interaction is the dominant separation mode. For some substances similar in hydrophobicity recognition capability may be poor, thus resulting in insufficient separation.
The COSMOSIL PYE reversed phase columns utilize various interactions based on p-electrons of the stationary phase. Its stationary phase is a planar pyrene or 2-(1-pyrenyl)ethyl) group bonded chemically to the silica gel. Utilizing such p-p interactions based on the pyrene ring, the PYE column separated substances similar in structure with ease.
totally porous spherical silica gel
Average particle size
Average pore size
Specific surface area
Charge transfer interaction
ANALYSIS OF MONO-SUBSTITUTED BENZENE
|Column: 4.6mm x 150mmMobile phase:5PYE :menthanol :water: 45:55|
|5C18-MS methanol:water 50:50Flow rate 1.0ml/min|
Detection UV 220nm, 0.6 AUFS
Sample 1. Benzene
2. Anisole 3. Nitrobenzene
Mobile phase: Toluene
Flow rate: 1.0 ml/min
Detection: UV 285nm
|Perfect separation of higher fullerenes on COSMOSIL PYE column.|
C18 columns have been commonly used for the separation of polycyclic aromatic hydrocarbons. However, their resolution for the separation of isomers is limited. The chromatogram below illustrates a separation of benz[a]anthracene and chrysene. Due to the electrostatic interaction between the sample and the pyrene ring in the stationary phase, the COSMOSIL PYE packed column can achieve the separation of aromatic isomers.
|Column: 5PYE 4.6mmx150mm|
Mobile phase: A:water, B:methanolGradient:B. 80%~100%, 20min linearFlow rate: 1.0ml/min
Detection UV 254nm,
16. Dibenz[ a,c]anthracene
17. Dibenz[ a,h]anthracen