Reversed-phase HPLC is the preferred separation method for the analysis of proteins and peptides, due to the superior resolution, high theoretical plate number, good reproducibility, ease of use and favorable cost performance.

In general, trifluoroacetic acid (TFA) is more often used as an acid mobile phase, in the analysis of proteins and peptides. However, reversed-phase HPLC using conventional silica gel shows some degradation over time in these very acidic conditions. The COSMOSIL AR-300 series packed columns have succeeded in improving acid resistance due largely to the development of a new synthesis method. It can be used for a longer time, while providing sharp peak shape even in the concentrations of 0.1% of trifluoroacetic acid.

Acid resistance

The COSMOSIL AR-300 series packed column show superior stability in the following decomposition test under the strong acidic conditions.
Capacity factor: toluene, Mobile phase :60% methanol

Types

Three alkyl and one aromatic type stationary phases are available in the COSMOSIL AR-300 series packed column. Please consult the table and applications below for selection guidance.
Alkyl type: 5C₁₈-AR-300, 5C₈-AR-300, 5C₄-AR-300 Aromatic type: 5Ph-AR-300

MATERIAL CHARACTERISTICS
	5C₁₈-AR-300	5C₈-AR-300	5C₄-AR-300	5Ph-AR-300
Silica gel	high purity spherical porous silica
Average particle size	approx. 300Å
Specific surface area	approx. 150m²/g
Stationary phase	octadecyl group	octyl group	butyl group	phenyl group
End capping treatment	near-perfect treatment
Carbon content	approx. 12%	approx. 7%	approx. 6%	approx. 7%

Semi-prep separation proteins

The sample loading capacity for protein or polypeptide separations is proportional to the cross section of column inside diameter. Using a short column, one can load a relatively large quantity of sample due to the absorption and concentration of sample inside the column. Below examples are shown for the separation using COSMOSIL 5C18-AR-300, with 4.6mmI.D. x 150mm column, 10mmI.D. x 150mm and 10mmI.D. x 250mm columns.
Identical separation patterns are achieved by adjusting proportionally the flow rate and the sample injection volume to the cross section of the column. There is little difference between 10mmI.D. x 150mm column and 10mmI.D. x 250mm column. Thus, semi-prep separations may be achieved using a shorter column.

Cosmosil 5C18 -AR-300 Column

Item	Catalog	Price ($U.S.)	Buy
5C18 -AR-300 – Semi-preparative Guard Column, 10 x 20 mm	093-7965	$371.00	Add to cart
5C18 -AR-300 – Analytical Column, 2.0 x 150 mm	093-7921	$422.00	Add to cart
5C18 -AR-300 – Analytical Column, 4.6 x 100 mm	093-7912	$453.00	Add to cart
5C18 -AR-300 – Analytical Column, 4.6 x 250 mm	093-7914	$525.00	Add to cart
5C18 -AR-300 – Analytical Column, 4.6 x 150 mm	093-7913	$422.00	Add to cart
5C18 -AR-300 – Analytical Column, 4.6 x 50 mm	093-7911	$268.00	Add to cart
5C18 -AR-300 – Analytical Guard Column, 4.6 x 10.0 mm	093-7910	$232.00	Add to cart
5C18 -AR-300 – Preparative Column, 10 x 150 mm	093-7917	$824.00	Add to cart
5C18 -AR-300 – Preparative Column, 20 x 150 mm	093-7919	$1,648.00	Add to cart
5C18 -AR-300 – Preparative Column, 20 x 250 mm	093-7920	$2,101.00	Add to cart
5C18 -AR-300 – Semi-preparative Column, 6x 250 mm	093-7916	$1,112.00	Add to cart
5C18 -AR-300 – Semi-preparative Column, 6 x 150 mm	093-7915	$1,071.00	Add to cart

Cosmosil 5C8-AR-300 Column

Item	Catalog	Price ($U.S.)	Buy
5C8-AR-300 – Analytical Column, 4.6 x 250 mm	093-7954	$525.00	Add to cart
5C8-AR-300 – Analytical Column, 4.6 x 150 mm	093-7953	$422.00	Add to cart
5C8-AR-300 – Analytical Column, 4.6 x 100 mm	093-7952	$453.00	Add to cart
5C8-AR-300 – Analytical Guard Column, 4.6 x 10.0 mm	093-7950	$309.00	Add to cart
5C8-AR-300 – Analytical Column, 4.6.x 50	093-7951	$330.00	Add to cart

Cosmosil 5C4-AR-300 Column

Item	Catalog	Price ($U.S.)	Buy
5C4-AR-300 – Preparative Column, 20 x 250 mm	093-8048	$2,369.00	Add to cart
5C4-AR-300 – Analytical Guard Column, 4.6 x 10 mm	093-7955	$247.00	Add to cart
5C4-AR-300 – Analytical Column, 4.6 x150 mm	093-7958	$494.00	Add to cart
5C4-AR-300 – Analytical Column, 4.6 x 50 mm	093-7956	$330.00	Add to cart
5C4-AR-300 – Analytical Column, 4.6 x 250 mm	093-7959	$618.00	Add to cart
5C4-AR-300 – Analytical Column, 4.6 x 100 mm	093-7957	$433.00	Add to cart
5C4-AR-300 – Semi-preparative Column, 10 x 250 mm	093-8047	$1,195.00	Add to cart

Cosmosil 5Ph-AR-300 Column

Item	Catalog	Price ($U.S.)	Buy
5Ph-AR-300 – Analytical Column, 4.6 x 100 mm	093-7962	$453.00	Add to cart
5Ph-AR-300 – Analytical Column, 4.6 x 150 mm	093-7963	$453.00	Add to cart
5Ph-AR-300 – Analytical Column, 4.6 x 250 mm	093-7964	$525.00	Add to cart
5Ph-AR-300 – Analytical Column, 4.6.x 50	093-7961	$330.00	Add to cart
5Ph-AR-300 – Analytical Guard Column, 4.6 x 10.0 mm	093-7960	$309.00	Add to cart

Mariana Defta

05:41 08 Sep 23

Thank you, Jessy Escalante, for your clear and immediate response

Renée Calpin

22:34 04 Sep 23

SES Research Inc has created some amazing products that I have absolutely loved trying and using! My vital c focus & energy has helped me to fall asleep faster and stay asleep longer, and my concentration has improved. ESS60 has such awesome studies and results I'm so glad I found this company. Their face serum is also doing wonders for my facial skin, it's moisturizing and I haven't had any pimples since I started using it! I definitely recommend this company and their products!

Daniel Stanberry

17:24 28 Aug 23

helped quickly and was clear in response. Thanks

Todd Walker

15:54 22 Aug 23

Breanna is great! Quick response and very helpful! Thanks!

Javi Hartenstine

20:31 20 Aug 23

Ron Mc Mahan

01:12 18 Aug 23

The ablity to quickly answer and correct address problems is great. Most places get almost at the end of the week, which is to late the order was shipped and billed wrong. So thanks for your help.

Frederic Sesma

22:26 17 Aug 23

Thank You for the fast response and resolve my subscription upgrade.

Donny L

16:45 04 Aug 23

Calling the toll free number listed on the website of SES Research was an example of excellence. Speaking with Breanna was Amazing. Not only was I able to immediately get in touch with a real person. The entire experience was seem less. Breanna was wonderful. Full of enthusiasm and life. Obviously Brianne is taking the miracle molecule. Very knowledgeable, professional and most of all genuinely helpful. Thank you Breanna and the entire SES Research team.

Luminita Jordache

16:45 12 May 23

very nice, friendly and competent advice, thank you for that, I will also buy from you in the future, I am very enthusiastic.The service I experienced was excellent Jessy tried very hard to send me the Carbon 60 order to Germany as quickly as possible I am very enthusiastic and I will be happy to order again in the future Greetings to Jessy. Until next time.

Donna

19:25 09 May 23

Breanna is amazing, knowledgeable and provides thoughtful customer service. She is truly committed to providing a great outcome of a bad situation. I have interacted with her to reorder and to help resolve missing orders. If I were her boss, she is the perfect example of the employee I would be looking to hire. Thank You Breanna for you excellent service and always having the customers back. SES is a great product and you have a great employee in Breanna.

koko sorgen

15:14 03 May 23

Been using C60 for months and the best benefit for myself is helping me to recover after workouts. It has helped my joints immensely. Also, great customer service! Received a bottle that had leaked and after contacting customer service with pictures and details, they are sending me a replacement.

Mariella Del Core

23:40 02 May 23

Breanna in customer service was a great help. She was very friendly in helping me make some changes to my account in no time. Breanna also followed up with an email to reassure any further help. I love the communication process!

DJ Wilson

20:17 30 Apr 23

Amazing customer service and outstanding products. I contacted CS several times and Breanna answered all my questions about original C60 and Skin Reduo. Very helpful, patient and kind! Love this company.

MrHipbkhop

21:33 19 Apr 23

I've used this product for a couple of yrs now and it's great I also put it in my face and it rejuvenates my skin. Best product for energy and over all organ health.

Brooke

01:06 14 Apr 23

Breanna answered my email very quickly. She was super helpful, knowledgeable, polite, and answered all of my questions. I wish all customer service reps were more like her. I wasn't left with any further questions as she was very detailed in her response. Kudos to you Breanna!! You went above and beyond!! 🙂

Carol Heinz

20:10 16 Mar 23

I am 83 years old and have been taking C 60 for about 2 1/2 years. The doctor I see once a year for yearly Medicare checkup says “I am one in a hundred”. I have no physical ailments and take no medication. (Of course, he does not know about my taking C 60.) my eyesight has actually been improving rather than getting worse with age and I can do many physical activities that my friends who are many years younger cannot.(I also recommended your C 60 product to three of my friends, who are now your customers.)

Ed Young

06:55 12 Feb 23

Wonderful product and super customer service. Joanna in CS was a great help, first in answering my email, and then in answering my phone call. There are generous and flexible product buying options not always mentioned in SES advertising emails that really show what a quality company SES works hard to be.

J Go

20:08 03 Feb 23

I have just recieved the product. I'm a cancer patient so I went with the higher quality olive oil. It does seem to have a decent amount of oleocanthal because it does have a peppery taste in the back of your throat. The taste is pleasant to me. For those who don't know, you only taste this upon swallowing.

John Tumelty

16:40 05 Dec 22

SES has succeeded in delivering a top quality product at a great price point. Not easy. Thank you Joanna for your deft and quick response to my questions + curiosity. As awareness grows about the power of c60 so will your brand and offering. Thanks for what you do. Love you guys!

Denis

04:54 02 Dec 22

I had a customer service question. Joanna explained what the solution was, and that I was not entitled to what I thought that I was. She did an excellent job.ThanksP.S. I do not expect to always be right. I only expect professionalism, and politeness in any explanation. That is what I got. Excellent customer service.

Jon Darcey

05:14 30 Nov 22

Outstanding customer service!Jessy is knowledgeable and finds the most cost effective solution to any queries I have.Apart from being the best product on the market it is matched by the best customer service.This is feedback for Breanna,I am astonished how the great customer service I received got even betterBreanna dealt brilliantly with a quite complex order. Hugely accomodating to my request.I'm not easily pleased but Breanna provided the best customer service I've ever received.Can't praise her enough. I know I'll receive exactly the same service again.

Andrew Beyer

20:52 23 Nov 22

First of all, SES is the top manufacturer of c60 products. They have been researching and producing fullerenes for 30 years, which is longer than any company currently in business. Their products were used in the original study that demonstrated the longevity benefits of c60, and their products continue to be used as reference samples and controls in ongoing research about c60 and c60 oil products. I am healing from a poorly understood chronic illness for which the best treatment option is lifestyle changes and antioxidant therapy. C60 is now my antioxidant of choice because I can feel monumental system-wide changes after only a week of daily c60 use, and I often notice the benefits of this compound within seconds of ingesting it. C60 is a true wonder drug as far as I’m concerned, and SES Research is the best source. Do your own research and you will find that Chris and his team are doing it better than anyone else. Big thanks to Joanna for great customer service, and thanks SES RES for the medicine that is helping to turn my life around.

Eva Tamez Treviño

01:15 16 Nov 22

I've been taking it for almost two months. I feel less tired and my sleep has improved.I had a big stock and wanted to put on hold my account. Joanna from customer service was very helpful and courteous helping me with this issue.

Bernard Chan

21:35 05 Oct 22

The C60 quality has been great and very well packaged. What was even more impressive and unexpected was the level of customer service (especially Breanna) - really prompt, patient and felt unscripted. Other companies take note, this is how you win long-term customers. One request, consider offering more no duty rate options (150ml and cases) up in Canada.

Bob Bradshaw

15:16 23 Sep 22

I started taking C60 in 2018 and it's really helped me with my overall health, especially mental clarity, sleep and joint pain. I purchase my C60 from SES Research in Houston, TX

Terri Williams

16:14 28 Jul 22

Jessy was very helpful in successfully creating for me a subscription for SES Carbon 60. He answered all my questions and was most friendly in doing so. I appreciate Jessy's help very much.

gail rinn

18:53 09 Jul 22

The service I've received has been excellent. Jessie and others have responded to my voicemail and email with prompt attention and information. This is something that can be hard to get these days!

Alex Kelley

02:35 27 Jun 22

Taking ESS60 in oil. I am liking this stuff, I am highly active in yoga and personal fitness and think it has been helping with recovery and more! Tastes great and feels great.

linda liguore

17:47 23 Jun 22

Joanna at SES customer service was awesome! I had purchased and tried C60 fine-grade olive oil with good results. My deep sleep score doubled when I began taking it (I measure sleep with an Oura ring). Sleep is so important to me, I was thrilled. A friend and Ph.D. researcher forwarded a published study that was done using the C60 molecule and olive oil resulting in unfavorable outcomes. I was concerned and sent a note to SES Research asking if they were aware of this study. Joanna responded within an hour pointing out in detail the discrepancies in protocols between the studies. Honestly, I didn't expect that I would hear back from SES Research. Her quick detailed response really up'd the score on my trust meter.

genevieve diamond

15:14 29 Apr 22

I contacted SES because one of the bottles in my shipment.was broken. SES's Jessy Escalante took care of the situation without any fuss, and my replacement bottle will be sent as agreed upon. SES--great product plus very helpful staff. Thank you, Jessy!

dale reynolds

16:56 28 Mar 22

Customer service is the best! I've had two problems with shipping and Joanna took care of them immediately no questions asked. I'd hope other companies would be to lucky to have her on their team, the best!Now I have tried SES before and their wonderful product DOES hold up to their advertisement on what it does, each time I take a dose it's like having comfort food, that warm fuzzy feeling you get, just love the feeling gained by using the product. My recommendation, You deserve the best in life especially for your body, SES will give you that. You'll have many health benefits, not only the highest grade product and totally bar none, Joanna will handle any problems that could arise, the best!My thanks to SES and Joanna at customer service, you're the greatest,Dale Reynolds

r m

22:06 08 Feb 22

In my knowledge this company has the highest rated product of its kind. C/S is helpful, knowledgable and efficient. I am a happy customer.

Jean-Paul Junod

19:29 15 Dec 21

I love the Carbon 60 that we purchase from SES Research. The flavor is great and while we don’t notice a big difference on a day-to-day basis, but we do realize a difference on the days where we don’t take it. We just don’t feel as limber and energetic. When we are sick, and it makes a HUGE difference.I also have received excellent customer service from Jesse. One of our bottles on auto ship came with a broken seal, and they are sending a replacement bottle. This company is also really good at delaying your auto ship for months out if you feel like you have too much product on hand. We’ve been ordering from SES Research for over a year now, and before that we were taking C60 that our natural health practitioner was making for over a year. We definitely feel like C60 from SES Research is better with the consistency and quality.

Daniel West

12:21 04 Dec 21

SES products are the best I have had nothing but positive responses from their products and the last product I ordered I received it in just two days. I really don't know how they do that, but it is fantastic, I would recommend their products to everyone...I had planned on living to the age of 100y/o but now with taking the SES-c60 they say it can possibly double your age so I'm shooting for 200y/o. You never know...Thank you SES Research Dan

Kalo Roma

22:15 19 Oct 21

SES Research is the original source of c60 in the US and they continue to offer a solid, quality product that it easy to dose correctly and use daily. Their customer support is very responsive and professional and will go the extra mile to ensure you have a positive experience. I recently had multiple issues with FedEx delivering my last order. Luckily, the SES representative stepped in on my behalf and coordinated things so that I was able to get my order delivered. It made all the difference. Hopefully they'll use UPS in the future rather than FedEx. Highly recommend their c60 in fine grade olive oil!

paola vergara

00:28 25 Sep 21

As a first time buyer I'm very pleased with the customer service . As I had some questions about the product and they got back to me fast and we're eager to help if I had any issues . I will continue use and reap the benefits . Thank you Jessy Escalante☺️

Edward Conroy

18:20 07 Jul 21

Jerry Escalante very readily and courteously helped me resolve a delivery product with my order, resulting in delivery that same afternoon after FedEx had said it was already delivered. Thanks, Jerry! I took my first dose last night and slept very well. Looking forward to tracking my progress! Thanks to the whole SES Research team for a great product, and great service. As I live in San Antonio, I'm happy to support a Houston, Texas-based business, too!

Calvin Casey

16:31 29 Jun 21

From the first phone call I made to inquire about this product to now - over a year later - they have been great! Someone picks up the phone, (no robots or "press this #) : ) They always get my order or requests correct the first time!! I wish more companies were like them. Thank you !

Cassidy Alexa

20:18 16 Oct 20

Jessy was very kind and helpful. I love the product. It gives me energy, calmness, improves my sleep, and overall well-being. I even love to put it on my skin. I give it to my dog as well, and he is energetic, his fur is velvet soft, and I have even noticed a small growth completely reverse. Thank you for being so transparent and honest. Best C60 around. Thank you again for the amazing customer support, and top notch quality.