Carbon-60 (C60 or fullerene) has been a subject of scientific fascination since its accidental discovery in 1985. Now, a groundbreaking study explores the potential of liposoluble C60, specifically dissolved in grape seed oil, as a potent anti-inflammatory and antioxidant agent. The study dives deep into its impact at the cellular, molecular, and whole-animal levels, shedding light on its mechanism of action.
Methods: At the cellular level, the study investigated the effects of liposoluble C60 on freshly isolated human neutrophils, focusing on key parameters like tumor necrosis factor-α (TNF-α) release, migration, phagocytosis, respiratory burst, and apoptosis. Molecular-level analysis involved studying the antioxidant effects of C60 dissolved in grape seed oil. Finally, at the animal level, the inhibitory effect of orally administered C60 on the inflammatory marker C-reactive protein (CRP) in beagle dogs was examined.
Results: The findings revealed that even trace amounts of C60 dissolved in grape seed oil significantly inhibited TNF-α release, cell migration, phagocytosis, and respiratory burst in human neutrophils. Moreover, C60 exhibited a notable scavenging effect on superoxide and 1,1-diphenyl-2-trinitrophenylhydrazine free radicals. Oral administration of C60 in grape seed oil markedly reduced CRP levels in beagle dogs, indicating a strong anti-inflammatory effect.
Discussion: The liposoluble nature of C60, previously insoluble in water, proved to be a pivotal factor in its effectiveness. The study suggested that C60-Oil could effectively enter neutrophils through cell culture fluid, inhibiting their inflammatory responses without inducing cytotoxicity. Additionally, C60 displayed antioxidant properties at the molecular level, scavenging superoxide and DPPH free radicals.
Conclusion: In conclusion, this study unveils the potential of hydrophobic C60 in hydrophilic media as a novel anti-inflammatory and antioxidant drug candidate. The results indicate that even in trace amounts, C60 in coconut oil can be transferred effectively to cells in water-soluble liquids, producing anti-inflammatory effects at the cellular level. This breakthrough holds promise for the development of new therapies targeting inflammation and oxidative stress, paving the way for future research in this exciting field.
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