Gel Filtration Chromatography

 

Gel filtration chromatography separates enzymes, proteins and other water- soluble polymers by utilizing the difference in the size of the molecules. Since mobile phases used for gel filtration chromatography are usually moderate buffer solutions or salts, this method can separate molecules while retaining their biological activity. The COSMOSIL ® Diol-IIpacked column is highly recommended for the separation of various water-soluble polymers.

 

Material characteristics
5DIOL-120-II
5DIOL-300-II
Silica gel
high purity spherical porous silica
Average particle size
5µm
Average pore size
approx. 120Å
approx. 300Å
Specific surface area
approx. 300m 2 /g
approx. 150m 2 /g
Stationary phase
Diol Group
Carbon content
approx.15%
approx. 9%

 

Selection of pore size
MW range
Flow rate(ml/min.)
protein
water soluble polymer
5DIOL-120-II
5,000-100,000
300-30,000
0.5-1.0
5DIOL-300-II
10,000-700,000
500-300,000


Go to Top

 

Cosmosil 5Diol-120 Column

Item Catalog Price ($U.S.) Buy
5Diol-120 – Guard Column, 7.5 x 50 mm 093-8049 $484.00 Add to cart
5Diol-120 – Packed Column, 7.5 x 300 mm 093-8050 $1,030.00 Add to cart
5Diol-120 – Packed Column, 7.5 x 600 mm 093-8051 $1,545.00 Add to cart

Cosmosil 5Diol-300 Column

Item Catalog Price ($U.S.) Buy
5Diol-300 – Packed Column, 7.5 x 600 mm 093-8054 $1,545.00 Add to cart
5Diol-300 – Packed Column, 7.5 x 300 mm 093-8053 $1,030.00 Add to cart
5Diol-300 – Guard Column, 7.5 x 50 mm 093-8052 $484.00 Add to cart