Gel filtration chromatography separates enzymes, proteins and other water- soluble polymers by utilizing the difference in the size of the molecules. Since mobile phases used for gel filtration chromatography are usually moderate buffer solutions or salts, this method can separate molecules while retaining their biological activity. The COSMOSIL ® Diol-IIpacked column is highly recommended for the separation of various water-soluble polymers.
|
Material characteristics
|
||
|
–
|
5DIOL-120-II
|
5DIOL-300-II
|
|
Silica gel
|
high purity spherical porous silica
|
|
|
Average particle size
|
5µm
|
|
|
Average pore size
|
approx. 120Å
|
approx. 300Å
|
|
Specific surface area
|
approx. 300m 2 /g
|
approx. 150m 2 /g
|
|
Stationary phase
|
Diol Group
|
|
|
Carbon content
|
approx.15%
|
approx. 9%
|
|
Selection of pore size
|
|||
|
–
|
MW range
|
Flow rate(ml/min.)
|
|
|
–
|
protein
|
water soluble polymer
|
|
|
5DIOL-120-II
|
5,000-100,000
|
300-30,000
|
0.5-1.0
|
|
5DIOL-300-II
|
10,000-700,000
|
500-300,000
|
|
Cosmosil 5Diol-120 Column
| Item | Catalog | Price ($U.S.) | Buy |
|---|---|---|---|
| 5Diol-120 – Guard Column, 7.5 x 50 mm | 093-8049 | $484.00 | Add to cart |
| 5Diol-120 – Packed Column, 7.5 x 300 mm | 093-8050 | $1,030.00 | Add to cart |
| 5Diol-120 – Packed Column, 7.5 x 600 mm | 093-8051 | $1,545.00 | Add to cart |
Cosmosil 5Diol-300 Column
| Item | Catalog | Price ($U.S.) | Buy |
|---|---|---|---|
| 5Diol-300 – Packed Column, 7.5 x 600 mm | 093-8054 | $1,545.00 | Add to cart |
| 5Diol-300 – Packed Column, 7.5 x 300 mm | 093-8053 | $1,030.00 | Add to cart |
| 5Diol-300 – Guard Column, 7.5 x 50 mm | 093-8052 | $484.00 | Add to cart |